duker, I agree that it would be worth going to the airport to find out, but quantifying the results may be difficult (observer effect).
I'll say this much, if you hold that you do less work walking up the down escalator than walking up an equivalent number of steps, then logically, I must assume that you also believe that walking down the up escalator would be harder than walking down an equivalent number of steps. If not, why not?
In any case, the extra work would come from the work you do against the treadmill (or escalator) motor. Every electrician, tech, and EE understands that a motor under load draws more current than one running no load. More current means more power. That's the reactive force against you propelling yourself at an upward vector (working against gravity).
Of course, this is all academic. Ultimately, I get on my treadmill and set the incline so that I feel a certain way when I do "treadmill hill repeats". If it doesn't feel like the real hills nearby, I adjust the treadmill (heck, I don't really know the precise % incline of the local hills to begin with).