Early in the year 2000, the LA Times quoted the WADA Director as saying about nandrolone metabolites in urine, "From what I understand, the medical data shows the only way you can go above the two-nanogram level [2 ng/ml of 19-NA] is by ingesting the drug. You can eat boar's testicles all week and not reach that level."
By 2008, WADA knew that these statements were erroneous based on a study they cite in their Technical Document. A single ingestion of boar offal could lead to a measurement of 130 ng/ml of 19-NA (nandrolone metabolite) in the urine -- 65 times more than what the WADA director thought was impossible. A single ingestion of boar meat (without the organs) could lead to 2.4 ng/ml of 19-NA, still more than the limit at that time. This study was only conducted on only three subjects so that the findings of 130 ng/ml for boar offal and 2.4 ng/ml for boar meat may not be even close to the upper limits had the study been conducted on 100 or 1,000 subjects. Houlihan had just 5.2 & 5.8 ng/ml of 19-NA in her urine samples. Somehow WADA convinced CAS arbitors that Houlihan had 2 - 3 times more 19-NA in her urine than ever seen in the scientific literature. It is easily provable with WADA's own citation that 30 times more 19-NA was seen in 1 of 3 subjects than found for Houlihan.
Also in the year 2000, the director of the Montreal laboratory involved in Houlihan's test (Ayotte), testified at a CAS hearing that the ratio of the two nandrolone metabolites, 19-NA and 19-NE showing an "equality of ratios" (similar amounts) was a hallmark of ingestion of a prohibited substance. The amounts of 19-NA and 19-NE for Houlihan were not similar as would have been expected (in the year 2000) for ingesting a prohibited substance. The ratio of 19-NA to 19-NE for Houlihan was 3.9 to 4.5. In the year 2000, this would have indicated that she was not taking a prohibited substance. But WADA had moved on to other problematic tests.
By 2006, Ayotte and others for WADA determined that one cannot use the 19-NA/19-NE ratio (as she testified to before CAS), but could tell the difference between exogenous nandrolone (doping) and endogenous nandrolone (naturally-occurring, including pork ingestion) by looking at the carbon isotope ratios using a GC/IRMS test. Figure 3 of Ayotte's 2006 report shows the exogenous (doping) results clustered between -27‰ and -31‰. The results in the report assumed to be endogenous (non-doping) were clustered between -19‰ and -23.5‰. Houlihan's measurement was -23‰. This would have indicated endogenous (non-doping) nandrolone metabolites. But as in the case of 19-NA and 19-NE ratios, WADA had moved on to other problematic tests.
Several years ago, WADA decided the way to differentiate exogenous nandrolone (doping) and endogenous nandrolone (non-doping) was to conduct the GC/IRMS test as mentioned above, but compare the results to a reference compound. However, like all of their other speculations, they did little testing as to the number of false positives that could be generated. As I pointed out in another post, one small study showed a 40% false positive rate and another more recent small study showed at least a 12.5% false positive rate when subjects ingested pork from uncastrated (farm-raised or wild) pigs.
Each time, WADA is so certain that their testing procedures are infallible and will testify to CAS as if they are. While there is no mention of pig diets in the WADA technical document, their experts relied on Houlihan's GC/IRMS test showing a result of -23‰ while pigs fed a diet of corn to soy ratio of 4 to 1 (and 10 to 1 for a finishing diet) will lead to human subjects with a GC/IRMS result of around -19‰. Any significant increase in the percentage of soy or addition wheat or barley or sorghum in the pig feed or any foraging by the pig can drastically change the result of the GC/IRMS test. Other grains like wheat are sometimes substituted to avoid the need for lysine and other supplements. The soy industry has been pushing farmers to use a higher percentage of soy meal. Soy carbon ratio: -27‰ ; Wheat: -25‰ to -27‰; Barley: -27‰ to -29‰. A pig feed with 4 to 1 corn to soy ratio will have a crude protein level of ~15% and a finishing feed with a ratio of 10 to 1 will have a crude protein level of ~7%. While the ingredient percentages are not listed on the label, one can see it is common for pig feed to have a much higher crude protein percentage (probably due to more soy or added wheat), especially finishing feed. The assumption by the WADA expert that all pigs are fed an imaginary average of 4 to 1 corn to soy (without any wheat, barley, sorghum, other ingredients or foraging) doesn't match with many products for sale and many recipies/articles available online.
Then there is the issue of meat contamination that wasn't mentioned. In 2021, Ireland found 10% of the pig samples with nandrolone, in Spain it was over 14% of the samples. In Austria it was only 0.66% and Poland only 1% of the pig samples with nandrolone. Malta had >18% of the pig samples with nandrolone in 2020. In 2017, WADA presented issues with meat contamination. They claimed that the chances of an adverse analytical finding was small in the EU and higher in certain hot spots. However, when tens of thousands of tests are conducted every year, it is likely there will be some cases of contamination leading to adverse analytical findings.
Finally, there is the issue that the WADA Technical Document that provided an alternate testing strategy for Houlihan's exact case. Houlihan's team asked that this testing strategy be followed as spelled out in WADA's own technical document, but the Athletics Integrity Unit ignored the request. I think that it is absurd for WADA to identify a problem, design an alternate testing strategy to address the problem, put all of that in their testing Technical Document and then have no way to trigger that strategy and then deny the need to use that strategy at a hearing. Was WADA's comments in their Technical Document just "random musings" rather than addressing a problem they identified? It appears so as those musings are still in their Technical Document with no mechanism for them being triggered.