Commonwealth Games champion Kaliese Spencer and World Championship relay medallist Riker Hylton have each been charged by the Jamaica Anti-Doping Commission (JADCO) with avoiding or refusing drugs tests.
The pair are alleged to have violated Article 2.3 of the JADCO Anti-doping Rules 2015 relating to "evading, refusing or failing to submit to sample collection".
Both athletes have already denied violating any rules.
Spencer was the the 400 metres hurdles champion at Glasgow 2014 and also formed part of a silver medal winning 4x400m relay team at the 2009 World Championships.
She told the Jamaican Observer that she will “pursue all legal channels necessary to protect her good name and her reputation as a true ambassador of Jamaicaâ€.
"This allegation is simply false, as Ms Spencer has always made herself available to be tested by JADCO," a statement provided by her management team added.