Wellnow,
Once again, you reveal how little you understand about what you are trying to discuss:
"Yes I know that the reactions of glycolysis are produced anaerobically in the cytosol. Why would you assume that I am ignorant about this? Because you don't read what I have written that's why."
Why? Read your very next statement:
"It's not really the point though is it? The terms anaerobic glycolysis and aerobic glycolysis do not make this distinction. They are a hangover from the time when lactate was thought to be only oxidizable after excercise cessation."
Nobody, ever, EVER, talks about aerobic glycolysis because glycolysis, by its very biochemical definition, is entirely anaerobic. Yet you keep throwing around the term "aerobic glycolysis" and are now calling it a hangover from another time. It is not a hangover, the term DOES NOT EXIST. Yet here you use it again right after crying about me pointing our your ignorance on metabolic biochemistry.
"Brooks explains why he believes other researchers have been unable to loctate LDH in the mitochondria:
http://jp.physoc.org/cgi/content/full/541/2/333
"
So HE says he found LDH in mitochondria, but two other labs didn't. Hardly conclusive evidence, huh?
"Yes pyruvate is a precursor to lactate production in the cytosol and oxidative phosphorylation in the mitochondria,
but that does not negate the argument that lactate production is an inevitable product of glycolysis in order to maintain cytosolic redox."
Explain why it is inevitable to maintain redox. This I want to hear from you.
"Why would we accumulate lactate at the same time as pyruvate was being exclusively the oxidizable glycolytic intermediate? that doesn't make sense. "
Sure it does. The oxidative pathway through pyruvate is running at max capacity, so the lactate pathway does what it does best by producing a quick yet inefficient energy boost. Lactate accumulates as a result. This is hardly a difficult concept to visualize.
"My own hypothesis (if you will allow a mere layman to think for himself) is that once lactate has been produced, the so called "anaerobic heat" has been produced and thus the muscles remain primed (warmed up) ready for more action in fight or flight with a ready supply of large amounts of energy, and with it the abundance of catecholamines necessary for this fight or flight. This is what we tap into when we interval train, it also explains why we feel so full of physical and nervous energy after a hard race or interval session, becasue we have to be, as do most other species in order to maintain our survival."
Here you go with this fuel source for "fight or flight". That insinuates a quick source of energy. AS I already discussed, lactate is produced AS A RESULT of a reaction that gives quick energy. Lactate must be recycled back, even if it is to produce energy aerobically, and it's already past the step where it can be used as a fast source of anaerobic energy. This is hardly a "fight or flight" adaptive mechanism, glucose will yield energy faster than lactate.
"You haven't provided any good evidence against Brooks' cell to cell or intracellular lactate shuttle hypotheses, which are accepted by Mader and Olbrecht as well as Noakes and many others and have been for more than a decade. "
Well, Noakes has been quoted on here showing he is a quack, and just because 1-2 others may like it there are a ton more that don't. As I mentioned, Brooks is trying to defend his position yet the majority of labs trying to verify his findings can't verify it.
"I think you are in denial spaniel, and your arguments are weak."
Only because you are too ignorant to understand half of what I say.